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MDNA may refer to:

• Mitochondrial DNA (mDNA or mtDNA), the DNA located in organelles called mitochondria
  • Mitochondrial DNA (journal), an academic journal
• MDNA (album), a 2012 album by Madonna
  • The MDNA Tour, the 2012 concert tour by Madonna to promote the album
    • MDNA World Tour (album), the live album/BD of the tour

• MDMA
• mRNA